1M5O
Transition State Stabilization by a Catalytic RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2002-03-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 259.380, 44.220, 102.500 |
| Unit cell angles | 90.00, 106.30, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1HP6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 57194 | |
| <I/σ(I)> | 22.9 | 1.9 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 300 | MPD, calcium Chloride, ammonium chloride, meta-ammonium vanadate, pH 5.0, VAPOR DIFFUSION, SITTING DROP at 300K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CaCl2 | ||
| 2 | 1 | 1 | NH4Cl | ||
| 3 | 1 | 1 | meta-ammonium vanadate | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | CaCl2 | ||
| 6 | 1 | 2 | NH4Cl | ||
| 7 | 1 | 2 | MPD |
