1M4U

Crystal structure of Bone Morphogenetic Protein-7 (BMP-7) in complex with the secreted antagonist Noggin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.04888, 0.91987, 0.91957, 0.88557
Spacegroup name	P 41 21 2
Unit cell lengths	99.834, 99.834, 150.327
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 * - 2.420
R-factor	0.24243
Rwork	0.241
R-free	0.27300 *
Structure solution method	MAD
RMSD bond length	0.035
RMSD bond angle	3.000 *
Data reduction software	TRUNCATE
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	MLPHARE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.483
High resolution limit [Å]	2.420	2.420
Rmerge	0.054 *	0.331 *
Total number of observations	92080 *
Number of reflections	28463
Completeness [%]	96.8	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.6 M disodium, 1.0 M monopotassium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	disodium	0.6 (M)
3	1	reservoir	monopotassium phosphate	1.0 (M)	pH6.5