1M43
Crystal structure of PMII in complex with pepstatin a to 2.4 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 1998-11-11 |
Detector | MARRESEARCH |
Wavelength(s) | 0.972 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 142.235, 142.235, 97.892 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.900 - 2.400 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sme |
RMSD bond length | 0.013 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.097 | |
Number of reflections | 35751 | |
<I/σ(I)> | 17.43 | 4.4 |
Completeness [%] | 79.5 | 62.2 |
Redundancy | 3.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |