1M2N
Sir2 homologues (D102G/F159A/R170A) mutant-2'-O-acetyl ADP ribose complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 84.720, 84.720, 193.087 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 * - 2.600 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.30600 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.500 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 * |
High resolution limit [Å] | 2.600 * |
Rmerge | 0.053 * |
Total number of observations | 81444 * |
Number of reflections | 19629 * |
Completeness [%] | 77.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | 18 * | PEG 8000, HEPES, pH 7.0, EVAPORATION, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mM) | |
2 | 1 | reservoir | sodium citrate | 100 (mM) | pH6.0 |
3 | 1 | reservoir | PEG4000 | 14 (%) | |
4 | 1 | reservoir | isopropanol | 20 (%) |