1LW3

Crystal Structure of Myotubularin-related protein 2 complexed with phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-20
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	66.080, 66.080, 261.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.25
Rwork	0.209
R-free	0.27200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m7r
RMSD bond length	0.006 *
RMSD bond angle	1.360 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.360
High resolution limit [Å]	2.300 *	2.280
Rmerge	0.051 *	0.288 *
Total number of observations	154888 *
Number of reflections	27735 *
<I/σ(I)>	20	5
Completeness [%]	92.0 *	73.8
Redundancy	7.5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	20 *	PEG 35000, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.0 (mg/ml)
2	1	drop	Tris-HCl	7 (mM)	pH8.0
3	1	drop		15 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	reservoir	PEG35000	3.0 (%)
6	1	reservoir	HEPES	100 (mM)	pH7.5
7	1	reservoir	dithiothreitol	2 (mM)