1LW3
Crystal Structure of Myotubularin-related protein 2 complexed with phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.080, 66.080, 261.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.25 |
| Rwork | 0.209 |
| R-free | 0.27200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m7r |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.360 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.360 |
| High resolution limit [Å] | 2.300 * | 2.280 |
| Rmerge | 0.051 * | 0.288 * |
| Total number of observations | 154888 * | |
| Number of reflections | 27735 * | |
| <I/σ(I)> | 20 | 5 |
| Completeness [%] | 92.0 * | 73.8 |
| Redundancy | 7.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | PEG 35000, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.0 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 7 (mM) | pH8.0 |
| 3 | 1 | drop | 15 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | PEG35000 | 3.0 (%) | |
| 6 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 7 | 1 | reservoir | dithiothreitol | 2 (mM) |
