1LQB
Crystal structure of a hydroxylated HIF-1 alpha peptide bound to the pVHL/elongin-C/elongin-B complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 58.940, 58.940, 243.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.000 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.27800 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1VCB.pdb |
RMSD bond length | 0.006 |
RMSD bond angle | 23.800 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 * | 0.490 * |
Total number of observations | 448657 * | |
Number of reflections | 24245 | |
<I/σ(I)> | 20 | 1 |
Completeness [%] | 76.0 | 30 |
Redundancy | 18.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 22 * | HEPES, PEG2000 monomethylester, ammonium sulfate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | reservoir | HEPES | 100 (mM) | pH7.4 |
3 | 1 | reservoir | PEG2000MME | 40 (%) | |
4 | 1 | reservoir | ammonium sulfate | 200 (mM) |