1LOA

THREE-DIMENSIONAL STRUCTURES OF COMPLEXES OF LATHYRUS OCHRUS ISOLECTIN I WITH GLUCOSE AND MANNOSE: FINE SPECIFICITY OF THE MONOSACCHARIDE-BINDING SITE

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (2.2 Å)

Cell axes56.300139.80062.700
Cell angles90.0091.0090.00
SpacegroupP 1 21 1
Resolution limits8.00 - 2.20
the highest resolution shell value -
R-factor0.179
R-work0.17900
RMSD bond length0.002 (0.003*)
RMSD bond angle3.000

Data Collection Statistics

Resolution limits - 2.20*
the highest resolution shell value -
Number of reflections33640*
Number of measurements77034*
Rmerge_l_obs0.082*
Completeness67.0*

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1Vapor diffusion, sitting drop*7.5*20**

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein10 (mg/ml)
21dropMPD40 (%(v/v))
31drop0.05 (M)
41dropHEPES0.02 (M)
Annotated Information is extracted from Literature Info*
Bourne, Y., (1988) J.Mol.Biol., 202, 685.