1LKI
THE CRYSTAL STRUCTURE AND BIOLOGICAL FUNCTION OF LEUKEMIA INHIBITORY FACTOR: IMPLICATIONS FOR RECEPTOR BINDING
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.100, 56.200, 95.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.186 |
Rwork | 0.186 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.480 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.092 * |
Completeness [%] | 90.0 |
Redundancy | 2.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6 * | 17 * | macroseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-15 (mg/ml) | |
2 | 1 | drop | MES | 10 (mM) | |
3 | 1 | reservoir | PEG8000 | 40 (%) |