1LK9
The Three-dimensional Structure of Alliinase from Garlic
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-04-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8423 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.450, 101.070, 155.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.530 |
Rwork | 0.193 |
R-free | 0.22100 * |
Structure solution method | SIRAS |
RMSD bond length | 0.011 |
RMSD bond angle | 2.200 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | MLPHARE |
Refinement software | REFMAC (4.0.6) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 1.560 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.057 * | 0.508 |
Total number of observations | 928820 * | |
Number of reflections | 160747 | |
<I/σ(I)> | 20.3 | 2.1 |
Completeness [%] | 98.9 | 99.6 * |
Redundancy | 5.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 4 * | Kuettner, E.B., (2002) Arch.Biochem.Biophys., 402, 192. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | HEPES | 5 (mM) | pH7.4 |
2 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
3 | 1 | reservoir | PLP | 20000 (mM) | |
4 | 1 | reservoir | 3 (mM) | ||
5 | 1 | drop | protein | 5 (mM) |