1L9U
THERMUS AQUATICUS RNA POLYMERASE HOLOENZYME AT 4 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-06 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 155.000, 271.200, 155.300 |
| Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 4.000 |
| R-factor | 0.345 * |
| R-free | 0.39700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.158 | 0.357 |
| Total number of observations | 107237 * | |
| Number of reflections | 85682 | |
| <I/σ(I)> | 5.4 | 1.8 |
| Completeness [%] | 79.9 | 70.1 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 22 * | HEPES, sodium formate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | HEPES-NaOH | 0.1 (M) | pH8.0 |
| 3 | 1 | drop | sodium formate | 3 (M) | |
| 4 | 1 | reservoir | sodium formate | 2.4-2.6 (M) |
