1KV5
Structure of Trypanosoma brucei brucei TIM with the salt-bridge-forming residue Arg191 mutated to Ser
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8500 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.943, 100.638, 109.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.650 |
R-factor | 0.143 * |
Rwork | 0.143 |
R-free | 0.17500 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tim |
RMSD bond length | 0.013 |
RMSD bond angle | 1.770 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.031 | 0.253 |
Total number of observations | 245458 * | |
Number of reflections | 60451 * | |
<I/σ(I)> | 37.7 | 5.4 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | ammonium sulfate, sodium chloride, citric acid, pH 5.5, VAPOR DIFFUSION, HANGING DROP at 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2 (M) | |
2 | 1 | reservoir | citric acid | 0.1 (M) | pH5.5 |
3 | 1 | reservoir | 0.2 (M) | ||
4 | 1 | drop | protein | 3 (mg/ml) | |
5 | 1 | drop | PGA | 10 (mM) |