1KO9
Native Structure of the Human 8-oxoguanine DNA Glycosylase hOGG1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 65 |
| Unit cell lengths | 105.513, 105.513, 47.597 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.070 - 2.150 |
| R-factor | 0.215 * |
| Rwork | 0.206 |
| R-free | 0.25800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ebm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 21.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.042 * | 0.250 * |
| Number of reflections | 17891 | |
| <I/σ(I)> | 13.4 | 3.5 |
| Completeness [%] | 99.9 | 100 * |
| Redundancy | 3.5 * | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 4.6 | 277 | PEG 4000, Ammonium Sulphate, Sodium citrate, pH 4.6, Microbatch, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG4000 | 27-30 (%(w/v)) | |
| 2 | 1 | 1 | ammonium sulfate | 0.2 (M) | |
| 3 | 1 | 1 | sodium citrate | 0.1 (M) | pH4.6 |
| 4 | 1 | 1 | protein | 10 (mg/ml) |
