Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1KLL

Molecular basis of mitomycin C resictance in streptomyces: Crystal structures of the MRD protein with and without a drug derivative

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X9B
Synchrotron siteNSLS
BeamlineX9B
Temperature [K]100
Detector technologyCCD
Collection date2000-05-15
DetectorADSC QUANTUM 4
Wavelength(s)0.9700
Spacegroup nameC 1 2 1
Unit cell lengths44.751, 60.458, 44.077
Unit cell angles90.00, 106.78, 90.00
Refinement procedure
Resolution10.000 - 1.500
R-factor0.124
Rwork0.131
R-free0.20900

*

Structure solution methodAB INITIO
RMSD bond length0.018
RMSD bond angle2.140
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareSHELXS
Refinement softwareSHELXL-97
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]25.0001.550
High resolution limit [Å]1.5001.500
Rmerge0.0460.301
Total number of observations66770

*

Number of reflections18054
<I/σ(I)>12.74
Completeness [%]99.9100
Redundancy3.643.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1unknown

*

6298ammonium sulfate, MES buffer, beta-octylglucoside, pH 6.0, VAPOR DIFFUSION, HANGING DROP at 298K, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
111protein3 (mg/ml)
211ammonium sulfate55 (%)
311MES100 (mM)pH6.0

217705

PDB entries from 2024-03-27

PDB statisticsPDBj update infoContact PDBjnumon