1KCQ
Human Gelsolin Domain 2 with a Cd2+ bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.200 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.968, 26.525, 50.294 |
| Unit cell angles | 90.00, 121.19, 90.00 |
Refinement procedure
| Resolution | 22.250 - 1.650 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Residues 151-266 from PDB ENTRY 1D0N |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.900 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 * | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.075 | 0.174 |
| Total number of observations | 44277 * | |
| Number of reflections | 13093 * | |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 99.0 | 94.6 |
| Redundancy | 3.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | PEG 400, cadmium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP at 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 50 (mM) | ||
| 2 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
| 3 | 1 | reservoir | PEG400 | 20 (%(v/v)) |
