1KC8
Co-crystal Structure of Blasticidin S Bound to the 50S Ribosomal Subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-27 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 212.902, 300.474, 575.176 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 3.000* |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NATIVE 1JJK |
| RMSD bond length | 0.009 |
| RMSD bond angle | 15.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.166 | 0.780 |
| Number of reflections | 361295 | |
| <I/σ(I)> | 12.5 | 2.5 |
| Completeness [%] | 94.5 * | 76.6 * |
| Redundancy | 7.4 * | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | back-extraction, vapor diffusion * | 5.8 | 292 | Ban, N., (2000) Science, 289, 905. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | NH4Cl | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 1 | NaOAC |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 1.2 (M) | ||
| 2 | 1 | reservoir | 0.5 (M) | ||
| 3 | 1 | reservoir | potassium acetate | 100 (mM) | |
| 4 | 1 | reservoir | 30 (mM) | ||
| 5 | 1 | reservoir | PEG6000 | 7 (%) | |
| 6 | 1 | reservoir | Tris | 15 (mM) | |
| 7 | 1 | reservoir | MES | 15 (mM) | |
| 8 | 1 | reservoir | 1 (mM) |
