1KBC
PROCARBOXYPEPTIDASE TERNARY COMPLEX
Experimental procedure
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.700, 80.800, 108.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.655 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.810 | 1.810 * |
| Rmerge | 0.066 | |
| Total number of observations | 150360 * | |
| Number of reflections | 35353 | |
| Completeness [%] | 96.6 | 96.7 * |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 22 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.3 (mg/ml) | |
| 2 | 1 | drop | Mes/NaOH | 206 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | 0.1 (mM) | ||
| 6 | 1 | drop | 0.04 (%) | ||
| 7 | 1 | drop | inhibitor | 6.6 (mM) | |
| 8 | 1 | drop | PEG6000 | 6.6 (%(w/v)) | |
| 9 | 1 | reservoir | sodium phosphate | 1 (M) |
