1K6Y
Crystal Structure of a Two-Domain Fragment of HIV-1 Integrase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2001-03-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2827 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 102.710, 102.710, 280.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.340 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.084 * | 0.356 * |
| Number of reflections | 53869 * | |
| Completeness [%] | 90.1 * | 84.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.2 * | 277 | 0.7 M NaH2PO4/1.0 K2HPO4, 0.1M Na acetate, pH 6.0, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | 0.5 (M) | ||
| 3 | 1 | drop | HEPES | 20 (mM) | pH7.2 |
| 4 | 1 | drop | 0.050 (mM) | ||
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | reservoir | 0.7 (M) | ||
| 7 | 1 | reservoir | 1.0 (M) | ||
| 8 | 1 | reservoir | acetate | 0.1 (M) | pH4.6 |
