1K6O
Crystal Structure of a Ternary SAP-1/SRF/c-fos SRE DNA Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1999-11-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0722 |
Spacegroup name | P 63 |
Unit cell lengths | 161.784, 161.784, 41.224 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.200 * |
R-factor | 0.27 |
Rwork | 0.220 |
R-free | 0.27570 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.175 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 |
High resolution limit [Å] | 3.200 * | 3.190 |
Rmerge | 0.131 * | |
Number of reflections | 10302 | |
Completeness [%] | 97.1 | 97.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 2000, pH 6.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 2000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | SAP-1 | 0.1 (mM) | |
2 | 1 | drop | SRF dimer | 0.1 (mM) | |
3 | 1 | drop | SRE DNA duplex | 0.13 (mM) | |
4 | 1 | drop | MES | 25 (mM) | pH6.0 |
5 | 1 | drop | PEG2000 | 8 (%) | |
6 | 1 | drop | 5 (mM) |