1K54
OXA-10 class D beta-lactamase partially acylated with reacted 6beta-(1-hydroxy-1-methylethyl) penicillanic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.649, 82.480, 101.957 |
| Unit cell angles | 90.00, 95.38, 90.00 |
Refinement procedure
| Resolution | 44.770 - 1.700 |
| R-factor | 0.17688 * |
| Rwork | 0.177 |
| R-free | 0.21043 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1e4d |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 14.341 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.770 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.064 | 0.289 |
| Total number of observations | 432971 * | |
| Number of reflections | 119631 | 16474 * |
| <I/σ(I)> | 12.9 | 3.2 |
| Completeness [%] | 99.1 | 93.8 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 4 * | ammonium sulafate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium potassium phosphate | 20 (mM) | pH7.8 |
| 3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5, or 100mM Na/Hepes(pH7.5) |
