1K3B
Crystal Structure of Human Dipeptidyl Peptidase I (Cathepsin C): Exclusion Domain Added to an Endopeptidase Framework Creates the Machine for Activation of Granular Serine Proteases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 1998-06-30 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 87.154, 88.031, 114.609 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.150 |
R-factor | 0.186 |
Rwork | 0.190 |
R-free | 0.23100 |
Structure solution method | MAD, MIR, MOL. REPL together |
RMSD bond length | 0.009 |
RMSD bond angle | 1.540 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | MAIN |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.180 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.070 | 0.249 * |
Total number of observations | 96833 * | |
Number of reflections | 23553 | |
Completeness [%] | 97.6 | 99 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | ammonium sulphate, sodium citrate, potassium/sodium tartrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
4 | 1 | reservoir | potassium/sodium tartrate | 0.2 (M) | pH5.6 |