1JWJ
Murine Inducible Nitric Oxide Synthase Oxygenase Dimer (Delta 65) with W457F Mutation at Tetrahydrobiopterin Binding Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-20 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 213.603, 213.603, 116.892 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.410 - 2.600 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nod |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 * | 0.450 * |
| Total number of observations | 142979 * | |
| Number of reflections | 46998 | |
| <I/σ(I)> | 15.1 | 2 |
| Completeness [%] | 97.2 | 87 |
| Redundancy | 3.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 4 * | Ammonium sulfate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | EPPS | 40 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 27-30 (%sat) | |
| 4 | 1 | reservoir | MES | 50 (mM) | |
| 5 | 1 | reservoir | BOG | 10-30 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 15-30 (mM) |
