1JT7
Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form with Amino Terminal His Tag AND LEU 44 REPLACED BY PHE AND LEU 73 REPLACED BY VAL AND VAL 109 REPLACED BY LEU (L44F/L73V/V109L)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 2001-02-22 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.540, 73.940, 108.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.900 - 1.700 |
R-factor | 0.192 * |
R-free | 0.23400 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jqz |
RMSD bond length | 0.007 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MRCHK |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.900 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.061 | 0.311 |
Total number of observations | 455187 * | |
Number of reflections | 71811 * | |
<I/σ(I)> | 31.5 | 3.1 |
Completeness [%] | 87.6 | 78.1 * |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 or 10 * | sodium formate, ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9-12 (mg/ml) | |
2 | 1 | reservoir | sodium formate | 3.4-4.4 (M) | |
3 | 1 | reservoir | ammonium sulfate | 1.0-1.2 (M) |