1JPP
The Structure of a beta-Catenin Binding Repeat from Adenomatous Polyposis Coli (APC) in Complex with beta-Catenin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.090, 69.255, 95.099 |
| Unit cell angles | 68.70, 87.04, 85.32 |
Refinement procedure
| Resolution | 28.270 - 3.100 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g3j |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.082 | 0.242 |
| Number of reflections | 19916 | |
| Completeness [%] | 90.7 | 87.8 |
| Redundancy | 1.47 | 1.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 20 * | PEG 8000, sodium potassium phosphate, sodium chloride, DTT, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 10 (%) | |
| 2 | 1 | reservoir | sodium potassium phosphate | 100 (mM) | pH6.2 |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | beta-catenin | 0.075 (mM) | |
| 6 | 1 | drop | APC-rA | 0.092 (mM) |
