1JNK

THE C-JUN N-TERMINAL KINASE (JNK3S) COMPLEXED WITH MGAMP-PNP

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	70
Detector technology	IMAGE PLATE
Collection date	1997-10
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	51.500, 71.240, 107.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.221 *
Rwork	0.222
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	24.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.300	2.300
Rmerge	0.052	0.165
Total number of observations	66063 *
Number of reflections	16394 *
Completeness [%]	90.0	75.4
Redundancy	4.03

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	20 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	AMP-PNP	1 (mM)
2	1	drop		2 (mM)
3	1	reservoir	mPEG550	18-20 (%(v/v))
4	1	reservoir	ethylene glycol	10 (%(v/v))
5	1	reservoir	beta-mercaptoethanol	20 (mM)
6	1	reservoir	HEPES	100 (mM)