1J7E
A Structural Basis for the Unique Binding Features of the Human Vitamin D-binding Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-02-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.912 |
Spacegroup name | P 43 |
Unit cell lengths | 131.977, 131.977, 73.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.510 - 2.550 |
Rwork | 0.213 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j78 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.410 |
High resolution limit [Å] | 2.370 | 2.370 |
Rmerge | 0.077 | 0.539 |
Number of reflections | 51580 | |
<I/σ(I)> | 15.9 | 2.1 |
Completeness [%] | 98.5 | 99.1 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | PEG 400, sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 20K |