1IVQ

THE CRYSTALLOGRAPHIC STRUCTURE OF THE PROTEASE FROM HUMAN IMMUNODEFICIENCY VIRUS TYPE 2 WITH TWO SYNTHETIC PEPTIDIC TRANSITION STATE ANALOG INHIBITORS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	32.200, 46.230, 135.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.600
R-factor	0.186
RMSD bond length	0.013
RMSD bond angle	0.039 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.600 *
Rmerge	0.095 *
Total number of observations	20722 *
Number of reflections	5285 *
Completeness [%]	86.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	6 (mg/ml)
2	1	drop	MOPS	0.1 (M)
3	1	drop	glycerol	10 (%)
4	1	drop	ethylene glycol	5 (%)
5	1	reservoir	PEG4000	25-30 (%)
6	1	reservoir	HEPES	0.1 (M)
7	1	reservoir	1-butanol	3-4 (%)