1ISS
Crystal Structure of Metabotropic Glutamate Receptor Subtype 1 Complexed with an antagonist
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-01 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.836 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 112.142, 112.142, 289.905 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.300 |
| R-factor | 0.257 * |
| Rwork | 0.257 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHAIN A of PDB ENTRY 1EWK |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.370 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.086 | 0.493 |
| Number of reflections | 27580 | |
| <I/σ(I)> | 1.6 | |
| Completeness [%] | 97.8 | 99.3 |
| Redundancy | 4.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Kunishima, N., (2000) Nature, 407, 971. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 0.2 (M) | pH8.5 |
| 2 | 1 | reservoir | ammonium sulfate | 1.9 (M) | |
| 3 | 1 | reservoir | S-MCPG | 10 (mM) |
