1IQD

Human Factor VIII C2 Domain complexed to human monoclonal BO2C11 Fab.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-10
Detector	ADSC QUANTUM 4
Wavelength(s)	1.10
Spacegroup name	P 21 21 21
Unit cell lengths	64.535, 73.701, 112.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.580 - 2.000
R-factor	0.203 *
Rwork	0.203
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.061 *
Total number of observations	284482 *
Number of reflections	36867 *
Completeness [%]	99.6	98.8
Redundancy	7.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	298	PEG8000, sodium chloride, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	10 (mM)
2	1	drop		0.1 (M)
3	1	drop	protein	10 (mg/ml)
4	1	reservoir	PEG8000	16 (%)
5	1	reservoir	HEPES	0.1 (M)
6	1	reservoir		0.2 (M)