1IKV
K103N Mutant HIV-1 Reverse Transcriptase in Complex with Efivarenz
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 270 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.02320 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 119.633, 157.168, 156.149 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.840 - 3.000 |
| R-factor | 0.229 * |
| Rwork | 0.228 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.840 | 24.840 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.152 | 0.630 |
| Total number of observations | 48782 * | |
| Number of reflections | 29152 | 2856 * |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 97.7 | 97.8 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.2 | 295 | ammonium sulphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | DMSO | 30 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
| 4 | 1 | reservoir | HEPES | 50 (mM) | pH7.2 |
| 5 | 1 | reservoir | 5 (mM) | ||
| 6 | 1 | reservoir | 300 (mM) |
