1HYS
CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH A POLYPURINE TRACT RNA:DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 115 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-03-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0909 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 166.160, 166.160, 218.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| Rwork | 0.274 |
| R-free | 0.31600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hmi |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.720 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.130 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.150 | 0.356 |
| Number of reflections | 56482 | |
| <I/σ(I)> | 12.2 | 2.9 |
| Completeness [%] | 82.0 | 65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 4 * | ammonium sulfate, cacodylate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulfate | ||
| 2 | 1 | 1 | cacodylate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | nucleic acid | 0.2 (mM) | |
| 3 | 1 | reservoir | cacodylate | 100 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 29-31 (%sat) |
