1HRN
HIGH RESOLUTION CRYSTAL STRUCTURES OF RECOMBINANT HUMAN RENIN IN COMPLEX WITH POLYHYDROXYMONOAMIDE INHIBITORS
Experimental procedure
Spacegroup name | P 21 3 |
Unit cell lengths | 141.100, 141.100, 141.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.800 |
R-factor | 0.215 |
Rwork | 0.215 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.700 |
Phasing software | X-PLOR |
Refinement software | TNT |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.800 * |
Rmerge | 0.066 * |
Total number of observations | 489730 * |
Number of reflections | 77612 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 * | enzyme solution resulted in a 4:1 molar excess * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | drop | inhibitor | 20 (mg/ml) | |
3 | 1 | drop | DMSO | 4 (%) | |
4 | 1 | reservoir | sodium citrate | 50 (mM) | precipitant |
5 | 1 | reservoir | 0.6 (M) | precipitant | |
6 | 1 | reservoir | PEG4600 | 20 (%(w/v)) | |
7 | 1 | reservoir | 0.02 (%) |