1HQV
STRUCTURE OF APOPTOSIS-LINKED PROTEIN ALG-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-09-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.04453 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 72.060, 72.060, 91.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
Rwork | 0.250 |
R-free | 0.29500 |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.220 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.061 | 0.213 |
Total number of observations | 69890 * | |
Number of reflections | 10911 | |
<I/σ(I)> | 23.7 | |
Completeness [%] | 96.8 | 96 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 8000, Calcium Acetate, Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
3 | 1 | reservoir | 100 (mM) | ||
4 | 1 | reservoir | PEG8000 | 12-15 (%) |