1HNZ

STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH HYGROMYCIN B

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (3.3 Å)

Cell axes402.063402.063175.263
Cell angles90.0090.0090.00
SpacegroupP 41 21 2
Resolution limits50.00 - 3.30
the highest resolution shell value3.420 - 3.300
R-factor0.218
R-work0.21800
the highest resolution shell value0.305
R-free0.26100
the highest resolution shell value0.321
RMSD bond length0.007
RMSD bond angle1.250 (28.500*)

Data Collection Statistics

Resolution limits99.00 - 3.30
the highest resolution shell value -
Number of reflections201587
Number of measurements630088*
Rmerge_l_obs0.158*
the highest resolution shell value0.385*
Completeness93.0
the highest resolution shell value83.7*
Redundancy2.9
the highest resolution shell value1.65
I/sigma(I)0

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1VAPOR DIFFUSION, HANGING DROP (unknown*)6.5277unknown*

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
111NH4Cl
211KCl
311CaCl2
411magnesium acetate
511sodium cacodylate
611MPD
712NH4Cl
812KCl
912CaCl2
1012magnesium acetate
1112sodium cacodylate
1212MPD
1313HYGROMYCIN B
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
412-mercaptoethanol6 (mM)
51potassium cacodylate0.1 (M )or 0.1M MES
Annotated Information is extracted from Literature Info*
Wimberly, B.T., (2000) Nature, 407, 327.