1HNE
Structure of human neutrophil elastase in complex with a peptide chloromethyl ketone inhibitor at 1.84-angstroms resolution
Experimental procedure
| Spacegroup name | P 63 |
| Unit cell lengths | 74.530, 74.530, 70.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 * - 1.840 |
| R-factor | 0.164 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.038 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.840 |
| Rmerge | 0.165 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 * | 20 * | referred to J.Biol.Chem. 262.17178-17181 1987 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 0.8 (M) | |
| 3 | 1 | drop | sodium acetate | 10 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 5 | 1 | reservoir | sodium acetate | 10 (mM) |
