1HF0
Crystal structure of the DNA-binding domain of Oct-1 bound to DNA as a dimer
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-04-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8856, 0.9185, 0.9190, 0.8424 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 131.250, 131.250, 116.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.2391 |
Rwork | 0.239 |
R-free | 0.29410 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.058 | 0.389 |
Number of reflections | 16742 | |
<I/σ(I)> | 23 | 3.5 |
Completeness [%] | 99.5 | 100 |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.3 | pH 5.30 |