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1HE8

Ras G12V - PI 3-kinase gamma complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID14-1
Synchrotron siteESRF
BeamlineID14-1
Temperature [K]100
Detector technologyCCD
Collection date2000-04-15
DetectorMARRESEARCH
Spacegroup nameP 31 2 1
Unit cell lengths113.635, 113.635, 183.881
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution52.030 - 3.000
R-factor0.212
Rwork0.212
R-free0.28000
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)PDB ENTRIES 1E8Y AND 5P21
RMSD bond length0.009
RMSD bond angle22.400

*

Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareCNS (1.0)
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0003.190
High resolution limit [Å]3.0003.000
Rmerge0.1020.301
Total number of observations232825

*

Number of reflections25822
<I/σ(I)>201.7
Completeness [%]91.650.5
Redundancy91.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

6.350 MM KH2PO4 PH 6.3, 4.3% PEG 8000
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropH-Ras0.170 (mM)
21dropPI3K0.029 (mM)
31reservoirpotassium phosphate50 (mM)pH6.3
41reservoirPEG80004.3 (%)

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PDB entries from 2024-03-27

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