1HCF

Crystal structure of TrkB-d5 bound to neurotrophin-4/5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-15
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	74.372, 80.406, 91.318
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.218
Rwork	0.218
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1B98 (NEUROTROPHIN) 1WWB (TRK)
RMSD bond length	0.007
RMSD bond angle	26.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.084 *	0.294 *
Number of reflections	15635
<I/σ(I)>	19.6	3.7
Completeness [%]	99.2	99.9
Redundancy	5.4	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5		10 MG/ML COMPLEX IN 20MM TRIS, 150MM NACL, PH 7.5, 100MM HEPES PH 7.5, 1.5M LISO4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	2		1.5-1.6 (M)
3	1	2	HEPES	100 (mM)	pH7.0-7.5