1H2T
Structure of the human nuclear cap-binding-complex (CBC) in complex with a cap analogue m7GpppG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-05 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 112.780, 112.780, 158.310 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.760 - 2.100 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.26600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h6k |
RMSD bond length | 0.006 |
RMSD bond angle | 20.400 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.210 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.079 | 0.398 |
Number of reflections | 60513 * | |
<I/σ(I)> | 5.3 | 1.8 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 | 277 * | Mazza, C., (2002) Acta Crystallogr.,Sect.D, 58, 2194. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 10 (%) | |
3 | 1 | reservoir | MES | 100 (mM) | pH6. |