1GS4
Structural basis for the glucocorticoid response in a mutant human androgen receptor (ARccr) derived from an androgen-independent prostate cancer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.350, 66.230, 73.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.500 - 1.950 |
| R-factor | 0.197 * |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e3g |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.020 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.500 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.045 | 0.266 |
| Total number of observations | 74323 * | |
| Number of reflections | 20515 * | |
| <I/σ(I)> | 18 | 4 |
| Completeness [%] | 99.9 * | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.2 | 20 * | pH 7.20 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.4 (M) | ||
| 10 | 1 | drop | dithiothreitol | 10 (mM) | |
| 11 | 1 | drop | protein | 1 (mM) | |
| 2 | 1 | reservoir | 0.4 (M) | ||
| 3 | 1 | reservoir | Tris-maleate | 0.1 (M) | pH9.5 |
| 4 | 1 | reservoir | PEG200 | 5 (%) | |
| 5 | 1 | drop | HEPES | 10 (mM) | pH7.2 |
| 6 | 1 | drop | n-octyl-beta-glucoside | 0.1 (%) | |
| 7 | 1 | drop | glycerol | 10 (%) | |
| 8 | 1 | drop | 9alpha-fluorocortisol | 10 (nM) | |
| 9 | 1 | drop | 150 (mM) |
