1GQF
Crystal structure of human procaspase-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 90.210, 90.210, 183.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.760 * - 2.900 |
| R-factor | 0.268 |
| Rwork | 0.268 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f1j |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.330 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.760 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.068 | 0.386 |
| Total number of observations | 180534 * | |
| Number of reflections | 19487 | |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.6 | 20 * | 50 MM MES, PH 5.6, 10 MM MAGNESIUM CHLORIDE, 100 MM AMMONIUM SULFATE, 20% (W/V) PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | procaspase-7 solution | 8 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate solution | 100 (mM) | |
| 3 | 1 | reservoir | MES | 50 (mM) | pH5.6 |
| 4 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
| 5 | 1 | reservoir | 10 (mM) | ||
| 6 | 1 | reservoir | PEG8000 | 20 (%) |
