1GOT
HETEROTRIMERIC COMPLEX OF A GT-ALPHA/GI-ALPHA CHIMERA AND THE GT-BETA-GAMMA SUBUNITS
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-05-10 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 133.400, 91.400, 83.200 |
Unit cell angles | 90.00, 120.10, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.196 * |
Rwork | 0.207 |
R-free | 0.29500 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.061 |
Number of reflections | 54174 |
Completeness [%] | 99.0 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | 10 MG/ML OF HETEROTRIMERIC COMPLEX WERE MIXED 1:1 WITH WELL SOLUTION CONTAINING 10% PEG-8000, 50 MM TRIS, PH 8.0, 10% GLYCEROL, 50 MM NACL, .1 MM MERCAPTOETHANOL. MIXTURE EQUILIBRATED VS WELL SOLUTION IN HANGING DROPS AT 4 DEGREES C., vapor diffusion - hanging drop, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
10 | 1 | reservoir | 50 (mM) | ||
11 | 1 | reservoir | beta-mercaptoethanol | 10 (%) | |
2 | 1 | drop | PEG8000 | 10 (%) | |
3 | 1 | drop | Tris-HCl | 50 (mM) | |
4 | 1 | drop | glycerol | 10 (%) | |
5 | 1 | drop | 50 (mM) | ||
6 | 1 | drop | beta-mercaptoethanol | 0.1 (%) | |
7 | 1 | reservoir | PEG8000 | 10 (%) | |
8 | 1 | reservoir | Tris-HCl | 50 (mM) | |
9 | 1 | reservoir | glycerol | 10 (%) |