1GKO

An Engineered Transthyretin Monomer that is Non-amyloidogenic - Unless Partially Denatured

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2000-12-15
Detector	M
Spacegroup name	P 21 21 21
Unit cell lengths	64.729, 83.301, 86.126
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.100
R-factor	0.22 *
Rwork	0.207
R-free	0.27550 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bmz
RMSD bond length	0.010 *
RMSD bond angle	1.540 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.170	2.060
High resolution limit [Å]	1.990	1.990
Rmerge	0.054	0.180
Number of reflections	27827
<I/σ(I)>	19.4	1.5
Completeness [%]	92.3	91.2
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	8.5 *		0.2 M MGCL2, 0.1 M TRIS BUFFER, PH 8.5, 30% W/V POLYETHYLENE GLYCOL 4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir		0.2 (M)
3	1	reservoir	Tris	0.1 (M)	pH8.5
4	1	reservoir	PEG4000	30 (%(w/v))