1G4U
CRYSTAL STRUCTURE OF THE SALMONELLA TYROSINE PHOSPHATASE AND GTPASE ACTIVATING PROTEIN SPTP BOUND TO RAC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2000-04-17 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 56.722, 58.260, 60.324 |
Unit cell angles | 70.03, 69.51, 65.03 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.106 | 0.427 |
Total number of observations | 177445 * | |
Number of reflections | 27264 | |
<I/σ(I)> | 13 | 3.7 |
Completeness [%] | 96.1 | 94 |
Redundancy | 5.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 18 %PEG 400, 0.1M MES, 15mM sodium flouride, 0.1mM aluminum chloride, 2mM DTT, 2mM magnesium chloride, pH 5.7, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG400 | 18 (%) | |
3 | 1 | reservoir | sodium fluoride | 15 (mM) | |
4 | 1 | reservoir | 0.1 (mM) | ||
5 | 1 | reservoir | dithiothreitol | 2 (mM) | |
6 | 1 | reservoir | 2 (mM) | ||
7 | 1 | reservoir | MES | 0.1 (M) | pH5.7 |