1G4U
CRYSTAL STRUCTURE OF THE SALMONELLA TYROSINE PHOSPHATASE AND GTPASE ACTIVATING PROTEIN SPTP BOUND TO RAC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.722, 58.260, 60.324 |
| Unit cell angles | 70.03, 69.51, 65.03 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.106 | 0.427 |
| Total number of observations | 177445 * | |
| Number of reflections | 27264 | |
| <I/σ(I)> | 13 | 3.7 |
| Completeness [%] | 96.1 | 94 |
| Redundancy | 5.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 18 %PEG 400, 0.1M MES, 15mM sodium flouride, 0.1mM aluminum chloride, 2mM DTT, 2mM magnesium chloride, pH 5.7, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG400 | 18 (%) | |
| 3 | 1 | reservoir | sodium fluoride | 15 (mM) | |
| 4 | 1 | reservoir | 0.1 (mM) | ||
| 5 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 6 | 1 | reservoir | 2 (mM) | ||
| 7 | 1 | reservoir | MES | 0.1 (M) | pH5.7 |
