1G1H
CRYSTAL STRUCTURE OF PROTEIN TYROSINE PHOSPHATASE 1B COMPLEXED WITH A BIS-PHOSPHORYLATED PEPTIDE (ETD(PTR)(PTR)RKGGKGLL) FROM THE INSULIN RECEPTOR KINASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.827, 90.112, 53.591 |
| Unit cell angles | 90.00, 99.21, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.207 * |
| Rwork | 0.207 |
| R-free | 0.24500 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.058 | 0.132 |
| Total number of observations | 105145 * | |
| Number of reflections | 16411 | |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 99.4 | 96 |
| Redundancy | 6.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | PEG 8000, MgCl2, dithiothreitol, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 302.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | C215A PTP1B | 10 (mg/ml) | |
| 2 | 1 | drop | peptide | 0.9 (mM) | |
| 3 | 1 | reservoir | PEG | 14-18 (%) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 6 | 1 | reservoir | 0.1 (0.1) |
