1FQ5
X-ray structure of a cyclic statine inhibitor PD-129,541 bound to yeast proteinase A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.5 |
Synchrotron site | SRS |
Beamline | PX9.5 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 87.300, 87.300, 110.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 12.900 - 2.400 |
R-factor | 0.2 |
R-free | 0.28000 |
RMSD bond length | 0.016 * |
RMSD bond angle | 0.040 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | AMoRE |
Refinement software | RESTRAIN |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.900 | |
High resolution limit [Å] | 2.400 | |
Rmerge | 0.071 * | 0.641 * |
Total number of observations | 50021 * | |
Number of reflections | 17302 | |
Completeness [%] | 89.6 | 81.2 * |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.5 | 298 | PEG 6000, Sodium Acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 10-25 (%(w/v)) | |
2 | 1 | reservoir | sodium acetate | 0.01 (M) | pH5.5 |
3 | 1 | drop | protein | 10 (mg/ml) |