1FOE
CRYSTAL STRUCTURE OF RAC1 IN COMPLEX WITH THE GUANINE NUCLEOTIDE EXCHANGE REGION OF TIAM1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9729,0.9787,0.9724 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.950, 149.270, 149.210 |
| Unit cell angles | 90.00, 121.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.262 * |
| Rwork | 0.262 |
| R-free | 0.29300 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.069 | 0.392 |
| Total number of observations | 382085 * | |
| Number of reflections | 80441 | |
| <I/σ(I)> | 27.9 | |
| Completeness [%] | 94.2 * | 68.9 |
| Redundancy | 4.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 | 4 * | PEG 3350, Li2SO4, MES, glycerol, pH 6.0, VAPOR DIFFUSION, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 100 (mM) | |
| 2 | 1 | reservoir | PEG3350 | 18 (%) | |
| 3 | 1 | reservoir | 400 (mM) | ||
| 4 | 1 | reservoir | glycerol | 5 (%) | |
| 5 | 1 | drop | protein | 20 (mg/ml) |
