1FD0

ISOTYPE SELECTIVITY OF THE HUMAN RETINOIC ACID NUCLEAR RECEPTOR HRAR: THE COMPLEX WITH THE RARGAMMA-SELECTIVE RETINOID SR11254

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-07-11
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	59.789, 59.789, 155.688
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.380
R-factor	0.131
Rwork	0.130
R-free	0.15800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fcy
RMSD bond length	0.013
RMSD bond angle	2.387 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.410
High resolution limit [Å]	1.380	1.380
Rmerge	0.042	0.362
Total number of observations	291021 *
Number of reflections	59004
<I/σ(I)>	36	3.3
Completeness [%]	99.8	99.8
Redundancy	4.9 *	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	17 *	sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP at 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PIPES	0.1 (M)	pH7.0
2	1	reservoir		0.3 (M)
3	1	drop	protein	3.7 (mg/ml)