1FCU
CRYSTAL STRUCTURE (TRIGONAL) OF BEE VENOM HYALURONIDASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1998-03-18 |
Detector | MARRESEARCH |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 71.157, 71.157, 152.064 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.100 |
R-factor | 0.209 |
Rwork | 0.200 |
R-free | 0.25700 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.028 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.100 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.111 | 0.326 |
Number of reflections | 23113 | |
<I/σ(I)> | 5 | |
Completeness [%] | 87.5 | 62.5 |
Redundancy | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 * | 20 * | PEG 8000, ammonium sulphate, Na cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium acetate | 5 (mM) | |
2 | 1 | drop | protein | 8-10 (mg/ml) | |
3 | 1 | reservoir | PEG8000 | 30 (%) | |
4 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
5 | 1 | reservoir | ammonium sulfate | 0.2 (M) |