1FB1
CRYSTAL STRUCTURE OF HUMAN GTP CYCLOHYDROLASE I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 115.110, 115.110, 387.310 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.970 - 3.100 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.29300 |
RMSD bond length | 0.011 |
RMSD bond angle | 23.800 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.970 | 3.290 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.165 | |
Total number of observations | 276838 * | |
Number of reflections | 25288 | |
Completeness [%] | 84.5 | 41.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | ammonium sulphate, isopropanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.6 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 100 (mM) | |
3 | 1 | drop | 1,4-dithiothreitol | 2 (mM) | |
4 | 1 | drop | 0.02 (%) | ||
5 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
6 | 1 | reservoir | isopropanol | 3.2 (%) | |
7 | 1 | reservoir | dithiothreitol | 2 (mM) |