1EZF

CRYSTAL STRUCTURE OF HUMAN SQUALENE SYNTHASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-11-01
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	76.760, 87.920, 154.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.150
Rwork	0.213
R-free	0.26200
Structure solution method	MIRAS
RMSD bond length	0.015
RMSD bond angle	1.590
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.042	0.141
Number of reflections	55224 *
<I/σ(I)>	35	7.2
Completeness [%]	96.0 *	84.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	25-30% PEG 4000, 0.1M sodium citrate, pH5.6, 0.2M ammonium acetate , VAPOR DIFFUSION, HANGING DROP, temperature 20K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1-2 (mg/ml)
2	1	drop	inhibitor		1:1 molar ratio
3	1	drop	reservoir		half-strength
4	1	reservoir	PEG4000	25-30 (%)
5	1	reservoir	sodium citrate	0.1 (M)
6	1	reservoir	ammonium acetate	0.2 (M)